59 Studies on Sulphatases 9 . THE ARYLSULPHATASES OF MAMMALIAN LIVERS
نویسندگان
چکیده
Kinetic data for the hydrolysis of potassium pacetylphenyl sulphate by fresh rat liver suspensions suggested that the arylsulphatase activity so observed could be attributed to a single enzyme (Dodgson, Spencer & Thomas, 1953) which was later found to be localized mainly in the microsomes of the liver cell (Dodgson, Spencer & Thomas, 1954a). Preliminary attempts to purify the microsome enzyme (unpublished data) were handicapped by its insolubility in various buffers and salt solutions of widely differing pH even after treatment of the liver by acetone-drying, alternate freezing and thawing, or use of the tissue disintegrator (Mickle, 1948). Sulphate and phosphate ions had little effect on the enzyme. These results contrast sharply with the arylsulphatase pattern reported for ox liver (Roy, 1953a, b; 1954). Using dipotassium 2-hydroxy-5nitrophenyl sulphate as the assay substrate, Roy was able to separate two arylsulphatases from the soluble material of acetone-dried ox liver and both enzymes were strongly inhibited by sulphate and phosphate ions. With the same substrate the arylsulphatase activity of mouse liver was found to be mainly concentrated in the mitochondria (Roy, 1953a). The present work, a preliminary report of which has already appeared (Dodgson, Spencer & Thomas, 1954 b), shows how the occurrence of three different arylsulphatases in mammalian livers enables these contrasting findings to be reconciled.
منابع مشابه
Studies on sulphatases. V. The determination of inorganic sulphate in the study of sulphatases.
متن کامل
Qualitative and Quantitative Analysis of the Effects of Quinazolinones on Internal Organs of Newborn Balb/C Mice
Objective(s) Quinazolinones are heterocyclic compounds, with biological and pharmacological activities, such as inhibiting some proteins, enzymes and reducing blood lipids. Materials and Methods Following previous results of our group, effects of two new derivatives of quinazolinones 9(3)-quinazolinone-2-propyl-2-phenylethyl (QPPE) and 9(3)-quinazolinone-2-ethyl-2-phenylethyl (QEPE) on liver...
متن کاملStudies on sulphatases. 4. Arylsulphatase and beta-glucuronidase concentrates from limpets.
متن کامل
Differential repression of arylsulphatase synthesis in Aspergillus oryzae.
1. The activities of the three arylsulphatases (arylsulphate sulphohydrolase, EC 3.1.6.1) of Aspergillus oryzae produced under a variety of repressing and non-repressing conditions were determined. 2. These enzymes exhibit different sensitivities to repression by inorganic sulphate. 3. Arylsulphatase I, but not arylsulphatases II and III, exhibits a transient de-repression in the early growth p...
متن کاملStudies on the nature of the multiple forms of the glutathione S-transferases.
various concentrations as a selective inhibitor and have found that ATP at a concentration of lOmM abolishes arylsulphatase B activity but only inhibits 50% of the arylsulphatase A activity. By measuring the total activity and the activity in the presence of 1OmM-ATP we have been able to determine the activity of both forms in defined mixtures of the two. Thus the proportions of the two enzymes...
متن کامل